NOTICE (July 15th, 2023): We are experiencing intermittent
issues connecting to the PolyPhen-2 webserver. If a run fails, we suggest to
wait a few minutes and resubmit the job. We apologize for the inconvenience.
update (Apr. 6, 2020): A paper describing an
application of Rhapsody to human ROMK (see Tutorial) has been published on PLoS Comput. Biol.
update (Feb. 26, 2020): The most recent paper describing Rhapsody's method and website has been published on Bioinformatics
update (Feb. 26, 2020): The most recent paper describing Rhapsody's method and website has been published on Bioinformatics
In silico saturation mutagenesis
perform a complete scan of all possible 19 amino acid substitutions at each site on a human sequence. See example: H-Ras.
Variants on custom PDB structure
perform saturation mutagenesis analysis on a specific conformer, homology model or homologous structure (in case human structure is not available).
Batch query of individual variants
obtain predictions for a batch of up to 10,000 individual human missense variants from various protein sequences. See an example here.